转录组测序分析猪胚胎附植的中期和后期卵巢差异表达基因

doi:10.11843/j.issn.0366-6964.2018.09.009

Abstract

Abstract:

Embryo implantation is one of the important factors affecting litter size in sows. In order to find the key genes regulating porcine embryo implantation. 2 Meishan sows selected and slaughtered on 18^th day and 24^th day of pregnancy,respectively followed by ovary tissues collection. And these tissues would be used in RNA sequencing (RNA-seq) analysis, GO analysis and pathway analysis.The results showed that:1) the most of sequences detected (reads) were gene exons in both tissues (ovary_18 d and ovary_24 d) during embryo implantation in pigs.The most reads were located in chromosome 6 (Chr 6) for ovary_18 d, followed by Chr 14, Chr 1 and Chr 7. For ovary_24 d, the most reads were located in Chr 1, followed by Chr 7, Chr14 and Chr M. In addition, there were more genes expressed and higher expression level in ovary_24 d than that in ovary_18 d. More than 60% genes of top 50 were located in mitochondria according to gene expression level, suggesting that mitochondria genes might play an important role in the regulation of porcine embryo implantation.2)581 genes were up-regulated and 103 genes were down-regulated in ovary_24 d compared with that in ovary_18 d. Among these genes, the expression difference of EN19684 was was highest, which was located in mitochondria, the expression difference of EN11278 was highest, which was located in Chr 14. 3)GO analysis results showed that these differentially expressed genes were significantly enriched in 38 biological processes such as endothelial cell activation, 19 cellular compartments such as host cytoplasm, and 16 molecular functions such as FAD-AMP lyase activity. Pathway analysis results indicated that these differentially expressed genes differed among 185 biological pathways, and the top 3 pathways were PI3K-Akt signaling pathway, Hippo signaling pathway and circadian cycle. In conclusion, genes expressed in ovary are mainly involved in the regulation of post-implantation, and high-expression genes are mostly located in mitochondria. The main function of differentially expressed genes is endothelial cell activation, and the main pathway of differentially expressed genes is PI3K-Akt signaling pathway.

CLC Number:

S828.2

FU Yan-feng, LI Lan, ZHAO Wei-min, FANG Xiao-min, LI Bi-xia, WANG Xue-min, ZHOU Li-sheng, REN Shou-wen. Differentially Expressed Genes Analysis between Mid-implantation and Post-implantation in Porcine Ovary by RNA Sequencing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1879-1888.

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Differentially Expressed Genes Analysis between Mid-implantation and Post-implantation in Porcine Ovary by RNA Sequencing

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